Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP) reveals common and dissimilar MP functions for tobamovirus spread.
Identifieur interne : 001D14 ( Main/Exploration ); précédent : 001D13; suivant : 001D15Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP) reveals common and dissimilar MP functions for tobamovirus spread.
Auteurs : Annette Niehl [Suisse] ; Adrien Pasquier [France] ; Inmaculada Ferriol [Espagne] ; Yves Mély [France] ; Manfred Heinlein [Suisse]Source :
- Virology [ 1096-0341 ] ; 2014.
Descripteurs français
- KwdFr :
- Arabidopsis (virologie), Corps d'inclusion viraux, Microtubules (métabolisme), Protéines de mouvement des virus de plantes (génétique), Protéines de mouvement des virus de plantes (métabolisme), Réticulum endoplasmique (métabolisme), Réticulum endoplasmique (virologie), Tobamovirus (génétique), Tobamovirus (physiologie).
- MESH :
- génétique : Protéines de mouvement des virus de plantes, Tobamovirus.
- métabolisme : Microtubules, Protéines de mouvement des virus de plantes, Réticulum endoplasmique.
- physiologie : Tobamovirus.
- virologie : Arabidopsis, Réticulum endoplasmique.
- Corps d'inclusion viraux.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Viral Movement Proteins.
- genetics : Tobamovirus.
- metabolism : Endoplasmic Reticulum, Microtubules, Plant Viral Movement Proteins.
- physiology : Tobamovirus.
- virology : Arabidopsis, Endoplasmic Reticulum.
- Inclusion Bodies, Viral.
Abstract
Tobacco mosaic virus (TMV) is a longstanding model for studying virus movement and macromolecular transport through plasmodesmata (PD). Its movement protein (MP) interacts with cortical microtubule (MT)-associated ER sites (C-MERs) to facilitate the formation and transport of ER-associated viral replication complexes (VRCs) along the ER-actin network towards PD. To investigate whether this movement mechanism might be conserved between tobamoviruses, we compared the functions of Oilseed rape mosaic virus (ORMV) MP with those of MP(TMV). We show that MP(ORMV) supports TMV movement more efficiently than MP(TMV). Moreover, MP(ORMV) localizes to C-MERs like MP(TMV) but accumulates to lower levels and does not localize to larger inclusions/VRCs or along MTs, patterns regularly seen for MP(TMV). Our findings extend the role of C-MERs in viral cell-to-cell transport to a virus commonly used for functional genomics in Arabidopsis. Moreover, accumulation of tobamoviral MP in inclusions or along MTs is not required for virus movement.
DOI: 10.1016/j.virol.2014.03.007
PubMed: 24889224
Affiliations:
- Espagne, France, Suisse
- Alsace (région administrative), Grand Est
- Illkirch, Strasbourg
- Université de Strasbourg
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Le document en format XML
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Its movement protein (MP) interacts with cortical microtubule (MT)-associated ER sites (C-MERs) to facilitate the formation and transport of ER-associated viral replication complexes (VRCs) along the ER-actin network towards PD. To investigate whether this movement mechanism might be conserved between tobamoviruses, we compared the functions of Oilseed rape mosaic virus (ORMV) MP with those of MP(TMV). We show that MP(ORMV) supports TMV movement more efficiently than MP(TMV). Moreover, MP(ORMV) localizes to C-MERs like MP(TMV) but accumulates to lower levels and does not localize to larger inclusions/VRCs or along MTs, patterns regularly seen for MP(TMV). Our findings extend the role of C-MERs in viral cell-to-cell transport to a virus commonly used for functional genomics in Arabidopsis. Moreover, accumulation of tobamoviral MP in inclusions or along MTs is not required for virus movement. </div></front></TEI><affiliations><list><country><li>Espagne</li><li>France</li><li>Suisse</li></country><region><li>Alsace (région administrative)</li><li>Grand Est</li></region><settlement><li>Illkirch</li><li>Strasbourg</li></settlement><orgName><li>Université de Strasbourg</li></orgName></list><tree><country name="Suisse"><region name="Grand Est"><name sortKey="Niehl, Annette" sort="Niehl, Annette" uniqKey="Niehl A" first="Annette" last="Niehl">Annette Niehl</name></region><name sortKey="Heinlein, Manfred" sort="Heinlein, Manfred" uniqKey="Heinlein M" first="Manfred" last="Heinlein">Manfred Heinlein</name></country><country name="France"><region name="Grand Est"><name sortKey="Pasquier, Adrien" sort="Pasquier, Adrien" uniqKey="Pasquier A" first="Adrien" last="Pasquier">Adrien Pasquier</name></region><name sortKey="Mely, Yves" sort="Mely, Yves" uniqKey="Mely Y" first="Yves" last="Mély">Yves Mély</name></country><country name="Espagne"><noRegion><name sortKey="Ferriol, Inmaculada" sort="Ferriol, Inmaculada" uniqKey="Ferriol I" first="Inmaculada" last="Ferriol">Inmaculada Ferriol</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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